GC-APCI II | Atmospheric Pressure Chemical Ionization

GC-APCI II Source Ultra-High Resolution TOF-MS on GC

Todays analytical chemistry often presents complex mixtures of compounds which are not available in libraries and databases. Identification of such components can be easily performed with Bruker’s ultra-high resolution TOF systems designed for LC or GC use.

With its second-generation GC-APCI source, Bruker offers the greatest flexibility and highest performance with a combined GC frontend and high-resolution MS system for confident ID of unknown compounds using complementary MS technology.

The GC-APCI II interface can be coupled to any Bruker MS system with the common Apollo II source design.

GC-APCI II is designed to connect to state-of-the-art GC systems including Bruker’s 436-GC and 456-GC but can also be used with third-party GC systems. The flexible, heated transfer line, automatic MS calibration, and outstanding sensitivity make GC-APCI II the best solution for unknown ID in metabolomics and small molecule research.

Target Applications

Unknown ID in Metabolomics

GC-MS is one of the few standard techniques in metabolomic analysis. In recent years the combination of gas chromatography and Atmospheric Pressure Chemical Ionization (APCI) with high resolution mass spectrometry techniques has proven to be an important additional tool for the analysis of highly complex mixtures of natural products from plants and body fluids. While standard GC-EI-MS is based on the availability of mass spectral libraries, GC-APCI allows the assignment and identification of unknown metabolites. GC-APCI II has arrived with improved performance, lower background and higher sensitivity; opening a new dimension in the analysis of metabolomic samples. 

Polycyclic Aromatic Hydrocarbons (PAH)

Polycyclic Aromatic Hydrocarbons (PAH) are ubiquitous in the environment and many of them are considered to be carcinogenic. The analysis of these compounds at low concentrations is therefore an important task in environmental and food analysis.

Atmospheric Pressure Chemical Ionization (APCI) in combination with high-resolution mass spectrometry techniques opens a new dimension in terms of sensitivity. APCI can be used in combination with GC and LC separation to cover the whole range of PAHs.

Dibenzodioxins and -furans

Dioxins are banned since the 1970s due to their ability to cause our springs to be silent. Their toxic and persisting nature still makes them compounds of both: Environmental and health concerns. The enormous toxicity of the “dirty dozen” requires highest sensitivity in the femtogram on column-range. Different chlorination pattern create technical mixtures of a few hundred congeners difficult to chromatographically resolve. GC-APCI can be ideally combined with hydrogen as carrier gas for highest chromatographic resolving power amongst all carrier gases.

Both environmental and food analysis can benefit not only for dioxin- and furan analysis but also other POPs like polychlorinated Biphenyls or brominated compounds.

GC-APCI II

Technical data

  1. Making a dedicated and expensive GC-TOF-MS used infrequently for just a few samples a thing of the past.
    The GC-APCI II source enables GC coupling to any Bruker TOF or QTOF, trap or FTMS system originally designed for LC coupling.
  2. Switching of the sources without venting the vacuum
    The system has the capability to be easily switched from GC to LC and vice versa without tools and within minutes. There is no need to vent the MS vacuum.
  3. Greater Flexibility
    The design of the flexible heated transfer line means that the GC and MS must no longer be precisely aligned. Interfacing the GC and MS is simpler, giving more options in positioning the MS and GC while preserving the obtained GC separation.
  4. Automatic MS Calibration
    The unique calibrant reservoir of the GC-APCI II interface enables software-controlled calibrant delivery.
  5. Improved LLOQ
    The optimized ionization chamber provides improved GC-MS performance including lower background and higher sensitivity.

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