High pressure analytical HPLC Autosampler
standard version of a fast and very versatile analytical HPLC autosampler, 1240 bar, 0.1-5000 µl injection volume
The Autosampler AS 6.1L can inject from up to 768 positions when equipped with microtiter plates (either high or low formats) or from up to 108 standard 1.5 ml sample vials. Sample carryover is significantly minimized thanks to a highly-effective interior and exterior needle wash procedure. This autosampler is also fast and flexible: one complete sample injection cycle takes less than one minute, including needle wash. Three different injection modes are supported; “full loop filling” (highest precision and reproducibility), “partial loop filling” (variable volumes, e.g. for dilution series) and “µl pickup” (loss-free injection of extremely small sample volumes), allowing the user to optimize sample usage. The headspace pressure function prevents bubbles from forming in the vial during sample uptake. Precolumn derivatization is supported. For high-pressure injections of up to 1240 bar, the autosampler is equipped with a so-called ILD™ valve (Intermediate Loop Decompression). This valve consists of a rotor-stator combination and includes a central port for depressurizing. For highpressure applications, the sample loop is depressurized prior to receiving the sample. This way, the sample is not diluted with solvent. Because the valve is switched extremely fast, pressure spikes are reduced. Analyses are more precise and wear of the column is reduced.
Sample Injection
| Autosampler Flow Path | Analytical |
|---|---|
| Maximum back pressure | 1240 bar |
| Vial/plate dimensions | well plate dimensions according to ANSI SLAS 4-2004 (formerly ANSI/SBS 4-2004) max. plate/vial height: 47 mm (incl. septa or capmat) |
| Injection volume range | 0.1 µl – 10 ml depending on sample loop |
| Sample loop | 10 µl |
| Dispenser syringe | 250 µl |
| Headspace pressure | built-in compressor, only for sample vials with septum |
| Switching time inj. valve | < 100 ms |
| Piercing needle precision | ± 0.6 mm |
| Sample tray cooling | none |
| Vial detection | missing vial/well plate detection by sensor |
| Needle wash | programmable: wash between injections and wash between vials |
| Wetted materials | Tefzel® (ETFE), Glass, Teflon® (PTFE), Kel-F® (PCTFE), stainless steel, PEEK, Vespel |
Specifications
| Power supply | 95–240 V AC |
|---|---|
| Dimensions | 361 x 375 x 570 mm (W x H x D) |
| Weight | 30 kg |
| Injection modes | full loop filling, partial loop filling and microliter pickup |
|---|---|
| Injection accuracy | 0.2 µl for 250 µl injection syringe |
| Injection precision | full loop filling: < 0.3 % RSD partial loop injection at injection volumes>5 µl: <0.5% RSD microliter pickup at injections>5 µl: <1.0% RSD |
| Sample carryover | < 0.0015 % for partial loop (chlorhexidine) < 0.0003 % with extended needle wash (s. Technical Note VTN0004) |
| Injections per vial | max. 9 injections |
| Injection cycle time | minimum 7 s from the same vial, 14 s from different vials;< 60 s for>100 µl sample injection in all injection modes, incl. 300 µl needle wash |
| Analysis time | max. 9 h, 59 min, 59 s |
| Inputs | 2 programmable TTL inputs (next injection, freeze, stop) |
|---|---|
| Outputs | 1 programmable relay output (inject marker, auxiliary, alarm) |
| Control | Ethernet (LAN) |
| Interfaces | LAN, analog |
