Simulated moving bed (SMB) chromatography saves time and money in the lab.
Simulated moving bed chromatography (SMBC) is increasingly applied as a separation technique in the pharmaceutical industry, production of fine chemicals, and in the field of bioengineering.
SMB is a method in process chromatography that enables substance mixtures to be continuously separated and extracted in two fractions. Its efficiency is significantly higher than batch chromatography, through better utilization of the column stationary phase.
Although in principle limited to two fraction separations, each partial fraction can be separated into further fractions by repeated use of the SMB process – down to binary substance mixtures.
Typically, the SMB process is used for two-component mixtures, like chiral separations or mixtures with a “leading” or a “trailing” key product peak in the chromatogram.
THE SMB PRINCIPLE
How it works
The SMB process enables the separation of binary mixtures by means of a simulated countercurrent between the solid and liquid phases. This is accomplished with a series of chromatography columns arranged in a ring. An eluent flow circulates through this ring. Two inlets (for feed and eluent) and two outlets (extract/red and raffinate/blue) define four separation zones. By continuously feeding sample and synchronously switching the columns against the eluent flow direction, a countercurrent is achieved between the solid and liquid phases, leading to high purity of both target fractions.
The movement of the solid phase is realized by simultaneously switching seven multi-position valves (AZURA® SMB) or one central multi-position valve (former KNAUER SMB, CSEP®). The bulk of the eluent is continuously circulated in the system, making it necessary to replace only that small amount which is removed in the extract and raffinate, thereby enabling savings of up to 90% of the eluent in comparison to a batch process. Due to the simulated countercurrent, the stationary phase is significantly better utilized with the SMB technique as compared to the batch process technique. The number of theoretical plates might be also less important, making it possible to use cost-effective larger particle size for the stationary phases.
SYSTEM SOLUTION FOR CONTINUOUS PURIFICATION
The multi-column lab-scale solution
The AZURA® Lab SMB System is optimized for the separation of binary mixtures in gram-scale. As with the AZURA Lab SMB System special emphasis is put on the continuous operation mode and high productivity. The SMB standard configuration consists of four AZURA Assistans ASM 2.2L with seven multiposition valves and four P 4.1S Pumps as well as our user-friendly software PurityChrom MCC and the required IT hardware. Referring to the special needs of every separation the SMB system can be freely configured (e.g. closed-loop, open-loop,) and is upgradable (e.g. detector, flow meter). A maximum pressure up to 130 bar with 8 columns is possible. Depending on your application the columns can be heated or cooled to ensure the perfect and most constant separation conditions. It can be chosen between a stainless steel or a bioinert version.
The multi-column system for pilot scale
The AZURA Pilot SMB System is optimized for the separation of binary mixtures in gram to kilogram-scale. As with the AZURA Pilot SMB System special emphasis is put on the continuous operation mode and high productivity. The SMB standard configuration consists of four Pump 2.1L and seven multiposition valves integrated into four AZURA Assistans ASM 2.2L. Our user-friendly software PurityChrom® MCC and the required IT hardware are included too. Referring to the special needs of every separation, the SMB system can be freely configured (e.g. closed-loop, open-loop) and is upgradable (e.g. detector, flow meter). A maximum pressure up to 100 bar (eight columns) is possible. Depending on your application the columns can be heated or cooled to ensure the perfect and most constant separation conditions.
Do you need more details or a quote?
We Can Help
Please send us your request.
We are happy to answer your questions about our products and services.