amaZon speed ETD
The new amaZon speed ETD incorporates key inventions in ion trap mass spectrometry for unprecedented proteomics performance.
Explicitly designed to enhance capabilities in research areas, compound screening and identification, and detailed chemical analysis.
The amaZon speed ETD incorporates key inventions for unprecedented proteomics capabilities. The high dynamic range and complexity of proteomics samples due to post-translational modifications require maximum speed and sensitivity to capture as many peptides as possible. The amaZon speed ion ETD trap sets new standards in protein identification due to its enhanced MS/MS speed, new SWIFT isolation and SMART fragmentation.
Structural confirmation and screening applications in routine labs are well covered, as are protein ID and detailed target characterization.


Key Features
- Bottom-up proteomics identifying more low abundant proteins due to its greatly increased MS/MS duty cycle
- Top-down sequencing (TDS) for the highest protein sequence coverage
- Market leading mass accuracy for confidence in results
- Leading dual ion funnel technology for unparalleled sensitivity
- Outstanding ETD/PTR applications for full post-translational modification (PTM) analysis
- SMART isolation and fragmentation
- Unrivaled scan speed of 52,000 u/sec at a peak width < 0.5 u for resolution of doubly charged ions
- High dynamic range
- Speed & sensitivity to capture as many peptides as possible.
- The amaZon speed ion ETD trap sets new standards in protein identification due to its
- Enhanced MS/MS speed
- New SWIFT isolation
- SMART fragmentation.
- Routine identification of more than 2500 unambiguously identified proteins in a single LC-MS/MS
- The dual ion funnel transfer line is delivering absolute benchmark sensitivity
Sensitivity
Leading dual ion funnel technology offers unparalleled sensitivity.
Confidence
Market-leading mass accuracy ensures high confidence in identification.
Speed
Unrivaled MS/MS scan speed unravels the complexity of proteomics samples.
Complete PTM characterization by Electron Transfer Dissociation (ETD)
Posttranslational modifications (PTMs)
Many proteins execute their molecular function by reversible posttranslational modifications (PTMs), like phosphorylation for example.
The identification and unambiguous localization of the modification site is of major importance to understand the biological context. ETD preserves the modification of the peptide backbone and therefore allows clear assignment of the modification site.
The amaZon speed offers unrivaled performance through its proven sensitive, robust, and reliable ETD technology.

Glycopeptide analysis

Peptide Sequence
Understanding relationships between structure, location, and function of glycoproteins requires detailed information about peptide sequence, glycan composition, and glycosylation sites. A wide variety of analytical approaches are employed to analyze intact glycoproteins, glycopeptides, and released glycans, with mass spectrometry playing a major role.
The performance of the amaZon speed ETD system for the analysis of N-glycosylated peptides in simple glycoprotein digest using Collision Induced Dissociation (CID) and Electron Transfer Dissociation (ETD) has been demonstrated.
The efficient combination of CID and oxonium-ion–triggered ETD during data acquisition – “Fragment Triggered ETD” – enables extensive protein sequence identification and detailed glycopeptide profiling in a single run, even in complex mixtures.
Combining the CaptiveSpray nanoBooster ionization source and Fragment Triggered ETD on the amaZon speed ETD platform greatly enhances glycopeptide analysis in protein mixtures.
System Components

ionBooster

GC-APCI II

Direct Insertion Probe (DIP)

APPI II

APCI II

