CHRONECT Symbiosis | Advanced

It offers all the advantages of online SPE to increase assay performance while reducing method development time and manual sample preparation steps.

SPE-LC and liquid injection without manual interaction

This instrument includes two SPE clamps that can run SPE cartridges (10 x 1 mm ID) up to 1,000 bar. With these clamps and the cartridge format, the system is optimally equipped to be used with UHPLC columns up to 1,000 bar and lower UHPLC flow rates than usual for LC separations. Method development is just as possible as with the PLUS system. Also, with ADVANCED, both direct UHPLC injection and online SPE injection can be performed without manual interaction. While sample purification is taking place on one cartridge, SPE-UHPLC-MS can run simultaneously on another cartridge. The run time of each sample is determined by the LC-MS analysis time. Peak focusing for the use of mixed-mode SPE cartridges at 1,000 bar can be realized with an additional SPH1299 UHPLC pump.

CHRONECT Symbiosis is controlled by CHRONOS Symbiosis 

CHRONOS organizes the connection to the LC-MS. All CHRONECT Symbiosis systems are able to process multiple batches/assays quickly and reliably with maximum analytical performance without supervision.

CHRONECT Symbiosis Advanced

Key Features

  • Fully automated and unattended switching between UHPLC-MS and SPE-UHPLC-MS
  • Simultaneous SPE sample cleanup and online SPE-UHPLC-MS analysis, reducing sample preparation time to zero
  • Standardized and fast method development
  • Easily optimized SPE method protocols that reduce LC-MS ionization suppression and sample carryover
  • LC peak focusing mode using mixed-mode SPE protocols for efficient and cleaner sample preparation
  • LC peak focusing mode (with additional UHPLC pump) using mixed-mode SPE protocols for efficient and cleaner sample preparation
  • Variable injection volume sample loops. Default: 100 µL
  • Automatic UHPLC solvent compressibility compensation and automatic purge and aspiration functions
  • Quickly wash the syringe, aspirate and dispense
  • Internal standard addition


Injection Direct UHPLC injection (LC mode)
ACE single cartridge clamp (SPE-LC mode)ACE dual cartridge clamp (SPE-LC mode): 1000 bar clamp & cartridges (10x1mm ID), 4 UHPLC valves
HPD Mix 1x SSM 7 SPE solvents to select
HPD syringe volume steps 10 µL to 2 mL
HPD SPE syringe flow rates 100 µL/min up to 10 mL/min
SPH1299 UHPLC gradient pump (binary gradient)1300 bar, including degasser, 2×2 solvent selection for four different binary gradients, automatic purge and prime valves
Mistral Cool column oven4 °C to 90 °C
Mistral CS Cool UHPLC5 columns plus drain, 4 °C to 75 °C, 1000 bar
Sample cooling with Peltier Stacks 2DW or 6DW2 or 6x 54 2 mL vials, 4 °C to 40 °C
Vortexer2 mL / 10 mL / 20 mL, one slot for custom vials, mixing with up to 2000 rpm
Agitator6 positions for 20 mL vials, adapters for 2 or 10 mL vials (optional), temperature range 40 to 100 °C, agitation speed 250-750 rpm
PAL3LSI, 1 tray plate with 3 racks VT54: racks for 3×54 2 mL vials
Injection Valves2 Valco injection valves
Syringe1 – 250 μL syringe (fixed needle, gauge 22, PST3, different syringes up to 1mL available)
Standard100 μL sample loop, various loops available
WashFast Wash module with pump and reservoirs for 2 solvents

High-performance solid phase extraction based on Spark technology
Standard configuration of all CHRONECT Symbiosis solution

 CHRONECT Symbiosis
Sketched Configurations


More than 400 publications, scientific posters and application notes can be found at

These documents cover various application areas, including:

  • Acid herbicides
  • Drugs in water
  • Pharmaceuticals in water
  • Microcystins in water
  • Pesticides
  • PAHs
  • Phenolic compounds
  • Carbamates
  • Antibiotics in water
  • Glyphosate in food
  • Aflatoxins
  • Glycoalkaloids
  • Chlormequat, mepiquat
  • Mycotoxins in foodVitamins

Many assays are being developed and there are EPA methods such as:

  • EPA 521 – Nitrosamines
  • EPA 535 –Acetamide
  • EPA 555 – Chlorinated acids
  • EPA 1694 – Pharmaceuticals
  • EPA 8270 – Semi-volatile substances

System Components

SPE Cartridges

SPE Cartridges

CHROspe SPE consumable, responsible for the efficient and reproducible extraction of analytes from samples, with maximum recoveries
Tool Change

Tool Change

Park Station and LC-MS Tool
DeCapper Module

DeCapper Module

This combination enables the complete automation of laboratory routines, e.g., the preparation of multistandard


Transferring liquids with pipettes is a common procedure in many types of sample preparation
2D Barcode Reader

2D Barcode Reader

Encoding sample information as a barcode is a standard tool in laboratory automation.
Wash Module

Wash Module

Fast Wash Module and Solvent Module
Efficient Mixing

Efficient Mixing

Vortex Mixer Module & Agitator Module
Sample Storage

Sample Storage

The CHRONECT Symbiosis Sampler offers you various options for sample storage: from simple trays for three racks each with vials of different sizes to drawers that can be cooled as required.


Liquid injection by the PAL is characterized by a combination of precision, reproducibility and flexibility.
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