CHRONECT Symbiosis | Basic

It minimizes manual sample preparation and gives you full traceability of the sample prep

This system offers all the basic advantages of online SPE to increase assay performance.

This system is the ideal starting point for fully automated sample preparation and LC-MS analysis, requiring only a minimum of manual preparation. Full traceability is provided by sample preparation documentation that is linked to the MS data. You can rinse the SPE cartridges to purify your sample as much as necessary and elute your analytes as long as required to the LC column, minimizing sample elution volume and time. The system is equipped with a single SPE Clamp. The preparation of each sample and the performance of the LC-MS analysis are performed sequentially. Sample run times are about 10 minutes – mainly depending on LC run times; SPE sample preparation is typically as little as 3 minutes. In addition, without reconfiguring the system hardware, you can decide whether to perform direct UHPLC injection or use extraction by an online SPE method. The system is upgradeable to higher throughput versions.

CHRONECT Symbiosis is controlled by CHRONOS Symbiosis 

CHRONOS organizes the connection to the LC-MS. All CHRONECT Symbiosis systems are able to process multiple batches/assays quickly and reliably with maximum analytical performance without supervision.

CHRONECT Symbiosis Basic

Key Features

  • Fully automated and unattended switching between HPLC-MS and SPE-HPLC-MS
  • Minimal or no manual sample pretreatment steps by using disposable SPE cartridges
  • Reduced LC-MS ionization suppression and sample carryover
  • Controlled sample preparation SPE flow rates (10-5,000 µL/min) with SPE pressures up to 250 bar with high-pressure dispenser (HPD)
  • SPE sample cleanup and online SPE-LC-MS/MS analysis in sequence
  • SPE sample preparation fully integrated with (U)HPLC-MS systems
  • Injection volume variable, standard: 100 µL
  • Automatic UHPLC solvent compressibility compensation and automatic purge and aspiration functions
  • Quickly wash the syringe, aspirate and dispense
  • Cost reduction of SPE solvents and sample collection vessels compared to manual SPE
  • Internal standard addition


Injection  Direct UHPLC injection (LC mode)
ACE single cartridge clamp (SPE-LC mode)  250 bar or 1000 bar clamp & cartridges, 1 UHPLC valve
HPD Mix 1x SSM  7 SPE solvents to select
HPD syringe volume steps  10 µL to 2 mL
HPD SPE syringe flow rates  100 µL/min up to 10 mL/min
SPH1299 UHPLC gradient pump (binary gradient) 1300 bar, including degasser, 2×2 solvent selection for four different binary gradients, automatic purge and prime valves
Mistral Cool column oven 4 °C to 90 °C

Specifications of options

Mistral CS Cool UHPLC 5 columns plus drain, 4 °C to 75 °C, 1000 bar
Sample cooling with Peltier Stacks 2DW or 6DW 2 or 6x 54 2 mL vials, 4 °C to 40 °C
Vortexer 2 mL / 10 mL / 20 mL, one slot for custom vials, mixing with up to 2000 rpm
Agitator 6 positions for 20 mL vials, adapters for 2 or 10 mL vials (optional), temperature range 40 to 100 °C, agitation speed 250-750 rpm


PAL Symbiosis standard specifications

PAL3 LSI, 1 tray plate with 3 racks VT54: racks for 3×54 2 mL vials
Injection Valves 2 Valco injection valves
Syringe 1 – 250 μL syringe (fixed needle, gauge 22, PST3, different syringes up to 1mL available)
Standard 100 μL sample loop, various loops available
Wash Fast Wash module with pump and reservoirs for 2 solvents



Special features of the
CHRONECT Symbiosis solutions

SPE cartridges

The online SPE cartridge for Symbiosis ACE systems has been specially developed for online elution to the HPLC column. The dimensions are optimized to combine high extraction capacity with small elution volumes. Standard are 10 mm length and 2 mm inner diameter. Of course, these cartridges resist the typical HPLC system pressures (max. 300 bar). All cartridges are also available in 10 x 1 mm; then the maximum pressure is 1,000 bar. These versions are manufactured with PEEK instead of PVDF. The materials for the sorbents are the same for both cartridge types.


Each cartridge is marked with a code indicating the brand, type and batch of SPE sorbent. In addition, 96 car-tridges each are provided in a rack that is inserted directly into the CHRONECT Symbiosis system for process-ing: No cartridge needs to be touched! Each tray contains an RFID chip that transmits cartridge type, lot number, production number (unique) and expiry date to Symbiosis. This information is displayed on the PC screen and is attached to the analysis report. After processing, the Symbiosis system transmits data about used/unused positions to the RFID chip. Racks can thus be stored safely without the need to label or remove used cartridges.


Method development

The systems are delivered with standard methods. These can be modified and adapted to the respective ap-plications at any time. When developing your own methods, many publications, application notes or scientific posters will help you.

Cartridge Packaging Program

A cartridge packaging program allows you to use your specific sorbents. In a first phase, it is possible to produce a smaller test batch with customer-supplied materials. This requires 10 g of sorbents for 25 cartridges. A safety data sheet must be available for the materials provided. If the results meet the expectations, a batch with at least 10 racks per order can be produced.

High-performance solid phase extraction based on Spark technology
Standard configuration of all CHRONECT Symbiosis solution


 CHRONECT Symbiosis
Sketched Configurations


More than 400 publications, scientific posters and application notes can be found at

These documents cover various application areas, including:

  • Acid herbicides
  • Drugs in water
  • Pharmaceuticals in water
  • Microcystins in water
  • Pesticides
  • PAHs
  • Phenolic compounds
  • Carbamates
  • Antibiotics in water
  • Glyphosate in food
  • Aflatoxins
  • Glycoalkaloids
  • Chlormequat, mepiquat
  • Mycotoxins in foodVitamins

Many assays are being developed and there are EPA methods such as:

  • EPA 521 – Nitrosamines
  • EPA 535 –Acetamide
  • EPA 555 – Chlorinated acids
  • EPA 1694 – Pharmaceuticals
  • EPA 8270 – Semi-volatile substances


System Components

SPE Cartridges

SPE Cartridges

CHROspe SPE consumable, responsible for the efficient and reproducible extraction of analytes from samples, with maximum recoveries
Tool Change

Tool Change

Park Station and LC-MS Tool
DeCapper Module

DeCapper Module

This combination enables the complete automation of laboratory routines, e.g., the preparation of multistandard


Transferring liquids with pipettes is a common procedure in many types of sample preparation
2D Barcode Reader

2D Barcode Reader

Encoding sample information as a barcode is a standard tool in laboratory automation.
Wash Module

Wash Module

Fast Wash Module and Solvent Module
Efficient Mixing

Efficient Mixing

Vortex Mixer Module & Agitator Module
Sample Storage

Sample Storage

The CHRONECT Symbiosis Sampler offers you various options for sample storage: from simple trays for three racks each with vials of different sizes to drawers that can be cooled as required.


Liquid injection by the PAL is characterized by a combination of precision, reproducibility and flexibility.
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