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Sensitive analytical HPLC with Online SPE sample preparation

Sensitive analytical system: Online SPE coupled to analytical HPLC

This dedicated system is specifically designed for solid phase extraction (SPE) in combination with a powerful analytical KNAUER AZURA HPLC system.

KNAUER’s versatile Assistant Module ASM 2.2L with two switching valves V 4.1 and a pump P 4.1S enables an efficient online SPE to concentrate analytes prior to HPLC analysis. This results in the reduction of analysis time and sample contamination as well as in an increase of analyte yield.
Afterward, separation and analysis can be achieved with a classical KNAUER AZURA analytical system equipped with P 6.1L LPG pump, a column thermostat CT 2.1 and a multi-wavelength detector MWD 2.1L to detect up to eight different wavelengths simultaneously.

This combination allows very sensitive determination of low concentrated samples and extracts e.g., bisphenol A (BPA) from water samples.

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Sensitive analytical HPLC with Online SPE sample preparation

Specifications

Application area HPLC
Pump system Gradient
Gradient Quaternary LPG
Injection Manual injection
Detector Variable multiwavelength detection UV/VIS
Software ClarityChrom
Pressure 862 bar
Column thermostat Yes

 

System Components

Multiposition valve, 12 Port

Multiposition valve, 12 Port

Multiposition valve, 12 ports, DLC stainless steel, PEEK, 1/8“, 100 bar, 1.5 mm bore size
Operated: electrical by VU 4.1
Port number: 12
Positions: Multiposition
Material: Stainless Steel
Capillary connection: 1/8"
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High-pressure injection valve, 6 Port

High-pressure injection valve, 6 Port

2-position valve, 6 Port, DLC stainless steel, Vespel®, 1/16", 1200 bar, 0.3 mm bore size
Operated: electrical by VU 4.1
Port number: 6
Positions: 2
Material: Stainless Steel
Capillary connection: 1/16"
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Customizable docking station for pumps, valves and detectors – ASM 2.2L

Customizable docking station for pumps, valves and detectors – ASM 2.2L

Left module: UV detector UVD 2.1S - EDA03XA Middle module: Valve drive VU 4.1 - EWA04 Right module: Pump P 4.1S, 10 ml, stainless steel - DPG22EA
Left module: UV detector UVD 2.1S - EDA03XA
Middle module: Valve drive VU 4.1 - EWA04
Right module: Pump P 4.1S, 10 ml, stainless steel - DPG22EA
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Versatile Multi Wavelength UV-Detector for Liquid Chromatography and Process Technology

Versatile Multi Wavelength UV-Detector for Liquid Chromatography and Process Technology

AZURA® Detector MWD 2.1L Multiwavelength detector MWD 2.1L, without flow cell 190 - 700 nm, incl. test cell
Detector type: Variable multiwavelength detector
Detection channels: 8 (Digital)/4 (Analog)
Wavelength range: 190 - 700 nm
Linearity : > 2.0 AU at 274 nm (ASTM E1657-98)
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Mounting bracket AZURA L

Mounting bracket AZURA L

Mounting bracket AZURA® L for KNAUER manual injection valves
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High-pressure manual injection valve, 6 Port

High-pressure manual injection valve, 6 Port

Manual injection valve, 6 ports, DLC stainless steel, Vespel®, 1/16“, 1200 bar, 0.3 mm bore size
Operated: Manual
Port number: 6
Positions: 2
Material: Stainless Steel
Capillary connection: 1/16"
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Chromatography Workstation for PurityChrom® and ClarityChrom®, PC with 24″ monitor

Chromatography Workstation for PurityChrom® and ClarityChrom®, PC with 24″ monitor

Desktop PC (SFF) for PurityChrom® and ClarityChrom® with 24" monitor, English edition Windows 10 Prof. 64-bit English, Intel® Core™ i5, 8 GB RAM, 256 GB SSD, two network cards
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Tool kit AZURA

Tool kit AZURA

Tool Kit AZURA® for systems with PEEK or pre-cut capillary kits
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Start-Up Kit with flexible, precut capillaries for analytical HPLC and UHPLC systems with 1/16″ connections

Start-Up Kit with flexible, precut capillaries for analytical HPLC and UHPLC systems with 1/16″ connections

Set of precut capillaries 0.18 mm ID, fittings, connectors and adapters, yellow
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WiFi router, 8x GBit RJ-45 ports, for connection of AZURA devices with a PC

WiFi router, 8x GBit RJ-45 ports, for connection of AZURA devices with a PC

WiFi router, 8x LAN GBit RJ-45 ports, 1x WAN GBit RJ-45 port
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ClarityChrom 8.7 – Workstation, autosampler control included

ClarityChrom 8.7 – Workstation, autosampler control included

ClarityChrom® single instrument license for one time base
Software name: ClarityChrom
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Analytical KNAUER PressureProof UV Flow Cell Cartridge

Analytical KNAUER PressureProof UV Flow Cell Cartridge

10 mm path length, 10µl, 1/16", 300 bar, PressureProof Flow cell cartridge for AZURA® Detector DAD/MWD
Flow cell type: Analytical UV flow cell
Path length: 10 mm
Capillary connection: 1/16''
Max. flow rate: 20 ml/min
Flow cell volume: 10 µl
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Column thermostat for up to 8 HPLC columns

Column thermostat for up to 8 HPLC columns

Column thermostat AZURA CT 2.1 for constant temperatures and reproducible results
Temperature range: 5–85 °C
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Low pressure gradient HPLC pump with 4 channel degasser

Low pressure gradient HPLC pump with 4 channel degasser

AZURA® Pump P 6.1L (LPG), with 10 ml pump head (stainless steel), degasser and mixer (200 µl)
Max. flow rate: 10 ml/min
Pump head materials: Stainless Steel
Gradient: Low-pressure gradient (quaternary)
Pump features: Active pulsation compensation, RFID for automatic pump head detection, Integrated pressure sensor, Purge valve (manual), Leak management, Piston seal wash
Maximum delivery pressure [MPa]: 86 MPa
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Black GL45 Safety Cap with air valve and fittings

Black GL45 Safety Cap with air valve and fittings

PTFE membrane keeps dust and other contaminants from the air
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AZURA Eluent tray E 2.1L

AZURA Eluent tray E 2.1L

AZURA® Eluent tray E 2.1L for AZURA® devices with a capacity of 6 x 1 l bottles or 4 x 2.5 l bottles or 2 x 5 l bottles, (delivery without bottles)
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