System for the analysis of aflatoxins, especially aflatoxin B1, B2, G1, and G2 in food

System Proposal

Sensitive, precise, and adaptable to your needs

Aflatoxins are the best-known group of mycotoxins produced as secondary metabolites by fungi, mainly by Aspergillus flavus and Aspergillus parasiticus, but to a smaller extent also by other strains. This origin is also where the name Aspergillus flavus toxin comes from. Aflatoxins can be produced on crops in the field or during storage of agricultural products, especially under warm conditions and high humidity. Unfortunately, these substances can persist long after the fungi have been killed and therewith contaminate foods. Most mycotoxins are stable compounds that are also not destroyed during food processing or cooking.

Since they can easily enter the marketplace and be a hazard to public health it is important to develop effective analytical methods for the identification and quantification of mycotoxins. Governmental institutions and health protection agencies apply these methods on a large scale to control marketed food products and animal feed. In the food processing industry the same methods are used to check raw materials and products, in order to direct them to countries with an appropriate legislation.

System for the analysis of aflatoxins B1, B2, G1, and G2 in food

Specifications

Application area HPLC
Pump system Gradient
Gradient Quaternary LPG
Injection Autosampler
Detector Fluorescence detection FLD
Software ClarityChrom, Mobile Control
Pressure 862 bar
Column thermostat Yes

The “AZURA® Aflatoxin System“ includes:

  • AZURA Autosampler AS 6.1L for automated sample injection
  • AZURA Pump P 6.1L low pressure gradient pump with maximum pressure of 862 bar
  • AZURA Column thermostat CT 2.1 for column tempering and reproducible results
  • Fluorescence detector RF-20A coupled to a photochemical postcolumn derivatization reactor for sensitive detection of aflatoxins
  • Sample preparation kit consisting of SPE cartridges and method protocol
  • Application description including method and analytical HPLC column

The system is controlled with ClarityChrom® software. Additionally, a tablet PC with AZURA Mobile Control to monitor the system status and for direct control can be used.

System Components

UVE UV-Derivatization Module

UVE UV-Derivatization Module

Photochemical reactor
Basic Fluorescence Detector for Liquid Chromatography

Basic Fluorescence Detector for Liquid Chromatography

Fluorescence detector RF-20 A 200 - 650 nm incl. accessories and flow cell
Detector type: Fluorescence detector
Detection channels: 1
Wavelength range: 200 - 650 nm
Interface Box IFU 2.1 LAN

Interface Box IFU 2.1 LAN

AZURA® Interface Box IFU 2.1 LAN, A/D converter, 4 channels
Tool kit AZURA

Tool kit AZURA

Tool Kit AZURA® for systems with PEEK or pre-cut capillary kits
Start-Up Kit with flexible, precut capillaries for analytical HPLC and UHPLC systems with 1/16″ connections

Start-Up Kit with flexible, precut capillaries for analytical HPLC and UHPLC systems with 1/16″ connections

Set of precut capillaries 0.18 mm ID, fittings, connectors and adapters, yellow
WiFi router, 8x GBit RJ-45 ports, for connection of AZURA devices with a PC

WiFi router, 8x GBit RJ-45 ports, for connection of AZURA devices with a PC

WiFi router, 8x LAN GBit RJ-45 ports, 1x WAN GBit RJ-45 port
ClarityChrom 8.7 – Workstation, autosampler control included

ClarityChrom 8.7 – Workstation, autosampler control included

ClarityChrom® single instrument license for one time base
Software name: ClarityChrom
Column thermostat for up to 8 HPLC columns

Column thermostat for up to 8 HPLC columns

Column thermostat AZURA CT 2.1 for constant temperatures and reproducible results
Temperature range: 5–85 °C
Autosampler AS 6.1L

Autosampler AS 6.1L

AZURA® Autosampler AS 6.1L analytical HPLC autosampler, 862 bar
Maximum back pressure: 862 bar
Sample loop: 100 µl
Sample tray cooling: None
Autosampler Flow Path: Analytical
Low pressure gradient HPLC pump with 4 channel degasser

Low pressure gradient HPLC pump with 4 channel degasser

AZURA® Pump P 6.1L (LPG), with 10 ml pump head (stainless steel), degasser and mixer (200 µl)
Max. flow rate: 10 ml/min
Pump head materials: Stainless Steel
Gradient: Low-pressure gradient (quaternary)
Pump features: Active pulsation compensation, RFID for automatic pump head detection, Integrated pressure sensor, Purge valve (manual), Leak management, Piston seal wash
Maximum delivery pressure [MPa]: 86 MPa
Black GL45 Safety Cap with air valve and fittings

Black GL45 Safety Cap with air valve and fittings

PTFE membrane keeps dust and other contaminants from the air
AZURA Eluent tray E 2.1L

AZURA Eluent tray E 2.1L

AZURA® Eluent tray E 2.1L for AZURA® devices with a capacity of 6 x 1 l bottles or 4 x 2.5 l bottles or 2 x 5 l bottles, (delivery without bottles)

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