Toxtyper® | Drug Screening

 

The Toxtyper® is a powerful push-button solution for rapid toxicology screening with a simplified, intuitive user interface

Drug screening simplified | Powerful UHPLC-MSn analysis

Toxtyper® delivers a robust and automated drug testing solution based on the fastest LC-MSn technology in the market, the amaZon speed. The Toxtyper® provides a combination of high-performance LC-MS/MS and confident identification with full transferability of library search results from lab to lab. The Toxtyper® was developed by toxicologists for toxicologists.

The Toxtyper® solution was developed to meet the needs of forensic and clinical research labs. The unique patented SmartFrag technology virtually removes any variation and hence the need for tuning from the MS/MS process.

The intuitive, easy-to-use Toxtyper® user interface is designed specifically for non-MS experts. Its push-button sample analysis is ideal for a multi-user environment and 24/7 operation.

Toxtyper®

Key Features

  • Tune-free, ready for use, robust LC-MSn solution for 24/7 toxicology screening
  • Easy to use software, designed for non-MS expert use
  • Comprehensive and confident screening results, based on high quality curated spectral libraries
  • Less than 12 minutes from injection to report, fast polarity switching for detection of basic, acidic and neutral compounds in one run
  • Allows for streamlined sample preparation – in particular for urine – without need for hydrolysis and/or derivatization
  • Open library concept enabling easy and fast library updates, e.g. for new psychoactive substances (NPS) in drug screening applications
  • Provides access to semi-quantitative results

The fastest automated drug testing on the market

Speed

Delivers an automated drug testing solution based on the fastest LC-MSn technology in the market.

Simplicity

User interface is designed for non-MS experts – push-button sample submission and analysis requires minimal user training.

Confidence

Compound ID based on MS, MS2 and MS3 spectra, plus retention time information, provides confident results.

Confident and comprehensive screening with simplicity and speed

Toxtyper® spectral libraries

The Toxtyper® holds a curated high-quality spectral library with approximately 3000 MSn spectra of nearly 1200 compounds of research toxicology interest. It also offers a smaller targeted library for drugs of abuse (DOA) screening research. The Toxtyper® supports the Maurer/Wissenbach/Weber LC-MSn library* containing almost 6000 drugs and metabolites.

Open library concept

Recent increases in drug abuse by new psychoactive substances (NPS), such as synthetic cannabinoids, constantly change their analytical requirements, meaning screening methods must be rapidly adapted.

The Toxtyper® libraries are constantly updated with contributions from customers and collaboration partners. User-defined library entries can be created quickly and easily, allowing laboratories to respond rapidly to new patterns of drug abuse.

Easy-to-use software designed for non-MS experts

The Toxtyper® software enables one-click review of library search results for fast interactive results evaluation using chromatogram and MSn spectra views.

The amaZon speed offers unrivaled performance through its proven sensitive, robust, and reliable ETD technology.

UHPLC combined with true MSn and comprehensive drug libraries

A complete solution for clinical research and forensic toxicology

Today’s forensic toxicology and clinical research labs are challenged by demands for fast and accurate results, along with lab efficiency and cost reduction. Further pressure results from the continuous emergence of new drugs into the market, requiring frequent updates of sample screening methods.

The Toxtyper® LC-MSn approach with library searching is an easy-to-use, flexible, and reliable screening solution for modern toxicology research.

Reproducibility and transferability through SmartFrag
Data-dependent MSn acquisition using Bruker’s patented SmartFrag technology enables the successful analysis of different types of compounds, regardless of their chemical nature, concentration, or sample matrix. The resulting MSn spectra and identification results are completely reproducible and transferable from instrument to instrument and lab to lab.

Toxtyper® – a complete toxicology solution

From comprehensive screening to semi-quantification:

Sample preparation

Different well-established sample preparation methods, such as liquid-liquid extraction (LLE), solid-phase extraction (SPE) or simple protein precipitation, can be used within the Toxtyper® workflow. Protein precipitation is quick and easy and is well suited to the comprehensive analysis of a wide range of analytes.

One example is to demonstrate the simultaneous, highly sensitive detection of drugs in human urine. Following simple sample preparation (liquid-liquid-extraction) of 1 mL of urine, the Toxtyper® permits detection down to 0.8 ng/mL.

Tailor-made sub-libraries for increased sensitivity

The Toxtyper® provides the option to generate smaller libraries for specific needs.

Screening, confirmation, and semi-quantitation in one run

To add value to the comprehensive screening, the standard Toxtyper® analysis using Toxtyper® and DOA libraries can be extended by semi-quantification.

After performing a one-point calibration for the target compounds using two or more internal standards, the subsequent Toxtyper® analyses provide qualitative screening results and semiquantitative values, e.g. concentration above or below a target value.

Toxtyper Case Study®
Analysis of drugs and toxins in the Katharinenhospital Stuttgart

Although drug analysis using immunological methods is fast, it is not always reliable. The available screening tests can easily lead to false positive results. For the confirmation of such immunoassay results, complex and time-consuming GC-MS analysis has been so far performed by the Stuttgart hospital.

Since March 2017, the Toxtyper® system has been used for the screening of drugs and toxins in blood, oral fluid and urine. Without time-consuming sample preparation, Toxtyper® delivers fast and reliable results that are highly specific and reproducible. In addition, the Toxtyper® provides a high degree of safety in toxicological analysis prior to the neurological diagnosis of irreversible brain failure.

Case Study

A 34-year-old patient with dizziness, nausea and vomiting was admitted to the emergency room. The anamnesis suggests consumption of drugs, but the patient denies the use of illegal drugs. Urine screening using various immunoassays was negative for Amphetamines, Barbiturates, Benzodiazepines, Cocaine, Opiates and Cannabis. The Toxtyper® identified Mitragynin and several metabolites of Paynanthein and Speciociliantin, which are alkaloids present in leaves and fruits of the Kratom tree. Kratom is used for substitution of Opioids and is not regulated by German narcotics law.

System Components

APPI II

APPI II

APPI is used for less polar or non-polar molecules that can not be ionized in either ESI or APCI.

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