Over time, instruments will wear, causing them not to run as smoothly or report results as accurately. Within this, part of the instruments can be replaced with new or refurbished spare parts, preventing downtime and keeping them working effectively.
Target Analysis’ repair services for GCs, HPLCs, mass spectrometers, and other lab instruments include warranty coverage, service agreements, and preventative maintenance. Whether you are seeking instrument repair or scheduling a service call for lab equipment repair.
Our experienced technicians will quickly restore your pump to top performance – regardless of its age. We offer comprehensive on-site support for vacuum components and systems, troubleshooting, maintenance, and repairs.
Target Analysis IQ/OQ/PQ procedures strictly follow good laboratory practices (GLP), which require that all laboratory instrumentation be inspected, cleaned, and maintained. We provide an independent and comprehensive testing service for Mass Spectrometry & Chromatography instruments to help you meet your regulatory and operational requirements and standards.
The philosophy behind our services is that we can support all the scientific instruments that we sell. Our technical department is trained and certified to offer a complete system service across multiple brands and models, including Mass Spectrometers (MS), Liquid Chromatographs (LC), Gas Chromatographs (GC), Autosamplers, Detectors & Peripherals
Remote support provides additional service through remote software to resolve instrument issues before a technician is dispatched. Our remote support team works with you via phone and software to remotely control your system to troubleshoot, repair or assist. This additional level of support allows for faster resolution of issues and ultimately reduces instrument downtime.
Application support is a service provided by our team that gives you a strategy and technique to quickly and confidently develop high-quality methods. You will learn a proven technique that will guide you step-by-step through the process of method development, applying principles that will make the process more efficient. Application support can take the form of a course, training, or development of an analytical method based on your real samples.
Omics technologies adopt a holistic view of the molecules that make up a cell, tissue, or organism. Target Analysis can provide unique solutions for bioinformatics services for proteomics and metabolomics workflows. Based on our experienced team members, we can readily meet the rigorous demands of various fields, such as life sciences, nutrition research, etc.
Customized training solutions from sample preparation to data analysis. Specially designed courses to meet today’s ever-increasing demands for analytical performance, productivity and ease of use!
Target Analysis was founded in 2013 by a team of experienced scientists and engineers.
Through the trust of our customers and partners, Target Analysis has managed to create a team with experienced and trained staff, which undertakes the installation of instruments, staff training, and after-sales support with spare parts, consumables, and certified reference materials. The company’s know-how is constantly expanding and is ready to take on any challenge to solve your analysis issues.
See how our customers are using Target Analysis’ products to transform their research and ultimately make discoveries that help change the way we see the future.
All-in-one software for compound identification from non-targeted workflows
Discover more biomarkers | With higher confidence
Identification of unknown compounds is supported through integrated tools, such as molecular formula determination based on accurate mass of precursors and fragments (SmartFormula3D), search of local and public databases (CompoundCrawler), in silico fragmentation to match theoretical to measured MS/MS (MetFrag) and MS/MS bucket matching for the identification of chemically related compounds.
Target Applications: metabolomics, lipidomics, phenomics, foodomics, environmental and pharma and provides users with the flexibility to support workflows ranging from basic ID to advanced statistics.
Spectral libraries for high confidence in compound identification
Identify & visualize
Add confidence to your IDs using annotation quality (AQ) scoring with CCS. Visualize biomarkers using built in statistical tools and map changing pathways.
CCS-enabled
Utilize a 4th dimension using TIMS to reveal CCS for all your compounds. Apply PASEF acquisition to trigger 10x more MS/MS events, enabling routinely higher confidence ID.
High throughput
Process large sample cohorts rapidly using MetaboScape’s client-server based software. Run > 200 samples per day using LC-free MRMS aXelerate.
SpatialOMx
Annotate imaging data with compound information, whilst detecting more compound classes using the innovative and unique MALDI-2 source on the timsTOF fleX.
Important Features to Consider
From acquisition to biological insight
MetaboScape® uses a unified workflow to process non-targeted analyses from Bruker’s ESI & MALDI Imaging instruments, simplifying the number of steps and rapidly pinpointing and identifying biomarkers.
MetaboScape® can be used across application areas, including discovery metabolomics, lipidomics, phenomics, foodomics, environmental and pharma and provides users with the flexibility to support workflows ranging from basic ID to advanced statistics.
MetaboScape®’s powerful T-ReX algorithm comprises retention time alignment, deisotoping and feature extraction to ensure robust data processing
Target compounds can be automatically annotated using user defined Analyte Lists
Unknown ID pipeline including library matching and in silico fragmentation to facilitate unknown ID
Visualize relevant information in complex data sets using supervised and non-supervised statistics, including PCA, t-test, ANOVA, PLS and bucket correlation analyses
Annotation Quality (AQ) scoring providing five indicators of data quality
Pathway mapping to set identified metabolites in a biological context, thereby turning data into knowledge
Identification of drug and xenobiotic metabolites using local metabolite prediction
Batch correction to offset sample effects in large sample cohorts
Time series plots to investigate changes in metabolites over time
Dedicated lipidomics annotation tools, including rule based annotation, 4D Kendrick mass defect plot and CCSPredict
To simplify the identification of knowns, MetaboScape® supports the MetaboBASE Personal Library, HMDB Metabolite Library, the Bruker Sumner MetaboBASE Plant Library (including CCS values for >130 compounds), as well as custom libraries
Customized data export to a file format suitable for import in GNPS (Global Natural Products Social Molecular Networking)
Client-server architecture to enable rapid data processing and multiple users to share methods and access shared datasets
Semi-targeted workflows in MetaboScape® go hand in hand with targeted workflows for absolute quantification using TASQ®
Benefits
Single workflow across platforms
The aim of non-targeted profiling is to identify features that are characteristic of a particular physiological state or sample. As there is no single workflow to enable access to the dynamic temporal and spatial fingerprints for all compounds, there is a need to evaluate data from complementary platforms. MetaboScape® addresses these needs by allowing for the evaluation of complementary data from both ESI and MALDI Imaging, as well as confidently assigning relevant markers in their biological context.
Identification of unknown compounds is supported through integrated tools, such as molecular formula determination based on accurate mass of precursors and fragments (SmartFormula3D), search of local and public databases (CompoundCrawler), in silico fragmentation to match theoretical to measured MS/MS (MetFrag) and MS/MS bucket matching for the identification of chemically related compounds.
Unknown ID pipeline:
A) SmartFormula3D limits possible precursor molecular formulea to typically one or a few candidates by automatically matching accurate mass and isotopic pattern fragment and precursor ion information.
B) Query of candidate formula in local and public databases returns possible structure candidates.
C) In silico fragmentation using implemented MetFrag functionality matches theoretical fragment structures to measured MS/MS peaks and scores most likely structure.
D) Optional MS/MS Bucket matching enables to assign compounds with similar MS/MS spectra for identification of further possibly unknown but likely structurally similar compounds.
Pharma workflows for identifying drug metabolites
Support for local BioTransformer based annotations in MetaboScape®. Generic Workflow for annotation of LC-MS/MS, LC-PASEF®, FIA-MRMS, MALDI Imaging
The identification of drug metabolites is not only of great interest to pharmaceutical research but has gained increasing interest in metabolomics, phenomics, exposomics and non-target screening workflows. Here, metabolites of drugs or other xenobiotics like pesticides, toxics or narcotics are expected to occur, which may belong to the family of unidentified, so-called dark metabolome compounds.
MetaboScape® supports a local BioTransformer1-based metabolite prediction for assignment of these metabolism products both from liquid samples and directly from tissue using the SpatialOMx workflow. Additionally, changes in time of these metabolites can be tracked and semi-quantified by using integrated time series plots.
([1] Djoumbou-Feunang et al.; Journal of Cheminformatics 2019, 11:2).
Fully integrated 4D-Lipidomics™ workflow
Rule based annotation routines in MetaboScape® enable the identification of lipid species taking into consideration the Lipidomics Standards Initiative (LSI) guidelines. This Lipid Class (LC) annotation tool avoids this risk of over annotation and simplifies the automatic identification of lipid features.
MetaboScape® can calculate and visualize Kendrick Mass Defects, turning complex mass spectral information into a compositional map with informative clustering of points based on lipid specific homologous repeating units (e.g. CH2). The customizable 4D Kendrick mass defect plot allows for intuitive lipid ID validation. Various characteristics of the extracted features can be plotted in 4 dimensions (x-axis, y-Axis, color scale, and bubble size), allowing versatile applications.
Plotting retention time vs m/z reveals the separation of different lipid classes using different colours for different lipid classes. Using this colour coding, you can easily spot annotations with obvious deviation in retention time or CCS relative to the rest of the same lipid class.
Plotting m/z vs CCS, you can further interrogate lipid data by visualizing trends in CCS observed for lipids with differences in chain length and double bond numbers. These trends can be used to confirm lipid class IDs and can assist in the annotation of unknowns and help to remove false positives.
Visualize the Kendrick Mass Defect with CH2 specified as repeating unit allows to quickly investigate lipid species of a selected class for saturation and chain length consistency. In addition, the shown example for lipids annotated as Triacylglycerols (TGs) reveals the expected elution order using reversed phase chromatography, as well as the increasing trends in CCS value making full use of all 4 complementary dimensions.
(Top) retention time vs m/z plot using different colours for different lipid classes and bubble size for the CCS values (Middle) m/z vs CCS, colour for retention time (Bottom) m/z vs KMD, bubble size for CCS; colour for retention time
Applications
T-ReX 4D – enabling 4D-OMICs
A major requirement of metabolomics and lipidomics analyses is to quickly pinpoint and identify those compounds that change as a result of perturbation or disease. Matching retention time, precursor mass, isotopic pattern and MS/MS spectra are common criteria for accessing confidence in compound annotations.
PASEF® data acquisition on the timsTOF Pro provides hundreds of MS/MS events per second, resulting in a greater depth of fragment coverage in single analysis. Additionally, PASEF® spectra benefit from ion mobility separation, therefore cleaner MS/MS spectra are obtained using an on-the-fly mobility filter. Each MS value is complemented with a collisional cross section (CCS) value to give a measure of the shape of the analyte, providing further confidence to ID.
T-ReX² and T-ReX³ for MALDI Imaging
In conjunction with SCiLS™ Lab software, T-ReX² empowers the SpatialOMx-based non-targeted profiling for processing and annotation of features, including drug metabolites, lipids and glycans. For the first time, map analytes spatially using this unique combination of T-ReX³ and combine it with CCS-enabled annotation of compounds to enable a higher confidence annotation of compounds acquired using MALDI Imaging on timsTOF fleX systems.
T-ReX² and T-ReX³ for MALDI Imaging
The chromatography free MRMS aXelerate workflow provides higher sample throughput by omitting time-consuming chromatography in phenomics research. Compounds are accessible that are not readily detectable by LC-MS analysis, allowing targeted and non-targeted metabolomics approaches. The data extraction by T-ReX 2D in MetaboScape® provides confidence in automatic annotation of the FIA MRMS data. The novel scimaX MRMS system can show its extreme performance with mass resolutions of >1 million and mass accuracies of <0.2 ppm. The ultra-high resolving power enables you to utilze isotopic fine structure for the unambiguous determination of elemental composition. This adds another layer of confidence for compound ID in non-targeted metabolomics.
