iST-BCT | For biological fluids

Don’t waste precious time preparing samples. Biological fluid sample preparation simplified!

Fast, reliable, reproducible sample preparation minimizing artificial modifications.

The challenge:

The multi-step methods of protein sample preparation for mass spectrometry analysis have inherent challenges of time, contamination and sample loss. When preparing biological fluids (e.g. plasma and serum) for proteomic analyses, harsh reagents may introduce artificial modifications resulting in reduced protein and peptide identifications.

The solution:

PreOmics’ iST-BCT sample preparation kit was developed to enable the robust processing of biological fluids and has been optimized to minimize artificial modifications (e.g. deamidation, oxidation) and increase the alkylation rate. Complex workflows previously requiring many different reagents over a two day time period are replaced by a single kit containing all standardized reagents to accomplish preparation for MS protein analysis.

Streamline workflows

1. Samples

Samples should be liquid, homogenates or a soft pellet.

2. Lyse, reduce, alkylate

Add LYSE reagent, and incubate for 10 mins at 95°C.

3. Digest

Cool, add DIGEST reagent, and incubate for 60 mins at 37°C.

4. Purify

Load onto cartridge, WASH and ELUTE. Dry and resuspend in LOAD.

5. MS analysis

Inject and analyze to see improved peptide identities.

iST-BCT Products

iST-BCT 8x

8 sample kit

iST-BCT 96x

96 sample kit | Racked into 96 well plate, has collars for individual processing

iST-BCT PreON 8x

8 sample kit | With PreON plasticware included

iST-BCT PreON 96x

96 sample kit | With PreON plasticware included


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