iST-BCT | For biological fluids
Don’t waste precious time preparing samples. Biological fluid sample preparation simplified!
Fast, reliable, reproducible sample preparation minimizing artificial modifications.
The multi-step methods of protein sample preparation for mass spectrometry analysis have inherent challenges of time, contamination and sample loss. When preparing biological fluids (e.g. plasma and serum) for proteomic analyses, harsh reagents may introduce artificial modifications resulting in reduced protein and peptide identifications.
PreOmics’ iST-BCT sample preparation kit was developed to enable the robust processing of biological fluids and has been optimized to minimize artificial modifications (e.g. deamidation, oxidation) and increase the alkylation rate. Complex workflows previously requiring many different reagents over a two day time period are replaced by a single kit containing all standardized reagents to accomplish preparation for MS protein analysis.
Samples should be liquid, homogenates or a soft pellet.
2. Lyse, reduce, alkylate
Add LYSE reagent, and incubate for 10 mins at 95°C.
Cool, add DIGEST reagent, and incubate for 60 mins at 37°C.
Load onto cartridge, WASH and ELUTE. Dry and resuspend in LOAD.
5. MS analysis
Inject and analyze to see improved peptide identities.
8 sample kit
96 sample kit | Racked into 96 well plate, has collars for individual processing
iST-BCT PreON 8x
8 sample kit | With PreON plasticware included
iST-BCT PreON 96x
96 sample kit | With PreON plasticware included