iST-Fractionation Add-on | Peptide fractionation
Don’t waste precious time preparing samples. Peptide fractionation simplified!
Simple, fast and reproducible peptide fractionation giving increased proteomic depth. Standardized methods for sample preparation – ready for mass spectrometry analysis
Preparing samples with complex proteomes e.g. human cell lines or tissues, or samples with a wide dynamic range in protein abundances for in depth proteomic characterizations is a compromise between effort, cost and method complexity.
The PreOmics iST-Fractionation Add-on is based on dipole-moment and mixed-phase interactions (patent pending). Samples are prepared using the PreOmics iST kits until the WASH step and then fractionated using PreOmics iST-Fractionation Add-on kit. The fractionation buffers are prepared ready for use and the method takes just 10 minutes. The resulting fractions provide a significant increase in the peptide and protein identifications from a single sample.
Follow iST or iST-NHS protocol until WASH steps.
Swap to a collection container. Add FRACTION-1 to the cartridge and spin.
Change to a new collection container. Add FRACTION-2 to the cartridge and spin.
Change to a new collection container. Add FRACTION-3 to the cartridge and spin.
5. MS analysis
Dry the samples and resuspend them in LC-LOAD. Inject and analyze to see improved peptide and protein identifications.
iST-Fractionation Add-on Products
8×3 reactions iST-Fractionation Add-on
8 sample kit
12×3 reactions iST-Fractionation Add-on
12 sample kit
96×3 reactions iST-Fractionation Add-on
96 sample kit