iST-NHS | Compatible with chemical labeling

Don’t waste precious time preparing samples. Chemically labeled sample preparation simplified!

Fast, reliable, reproducible sample preparation for chemical labeling (iTRAQ and TMT).

The challenge:

Chemical labeling reagents have specific handling and compatibility requirements to achieve good labeling efficiencies. Combined with the multi-step methods of proteomics sample preparation for mass spectrometry analysis, chemical labelling protocols have inherent challenges of time, contamination and sample loss. This challenge is exacerbated with large numbers of samples and small starting amount of input material.

The solution:

PreOmics iST-NHS sample preparation is fully compatible with chemical labeling reagents. Allowing streamlined peptide processing, chemical labeling and an efficient peptide clean-up step. The iST-NHS method decreases hands-on time, sample loss and the amount of chemical label required. Complex workflows previously requiring many different reagents over a two day time period are replaced by a single kit containing all standardized reagents to accomplish proteomics sample labeling and preparation for MS protein analysis.

Streamline workflows

1. Sample

Samples should be liquid, homogenates or a soft pellet.

2. Lyse, reduce, alkylate

Add LYSE reagent, and incubate for 10 mins at 95°C.

3. Digest and label

Cool, add DIGEST reagent, incubate for 60 mins at 37°C. Label according to vendor instructions.

4. Purify

Load onto cartridge, WASH and ELUTE. Dry and resuspend in LOAD.

5. MS analysis

Inject and analyze to see improved peptide identities.

iST-NHS Products

iST-NHS 12x

12 sample kit

iST-NHS 96x

96 sample kit | Racked into 96 well plate, has collars for individual processing

iST-NHS HT 192x

192 sample kit | Reagents in amber bottles for easy use with liquid handlers

iST-NHS PreON 12x

12 sample kit | With PreON plasticware included

iST-NHS PreON 96x

96 sample kit | With PreON plasticware included


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