iST-Trap | GFP immunoprecipitation preparation
Don’t waste precious time preparing samples. Immunoprecipitation sample preparation simplified!
Fast, reliable, preparation from immunoprecipitated samples ready for mass spectrometry.
Integrating immunoprecipitation with multi-step methods of proteomics sample preparation for mass spectrometry analysis has inherent challenges of time, contamination and sample loss. Preparing labelled samples over extended period with a complex method leads to variation in results especially when working with high sample throughput and small starting amounts of input material.
The PreOmics / ChromoTek iST-Trap Kit enables the fast and efficient immunoprecipitation of GFP-Fusion proteins and the interaction factors followed by the innovative sample preparation for mass spectrometry based proteomics. All in less than 4 hours.
1. Sample immunoprecipitation
Samples should be liquid, add GFP-Trap_A and incubate at 4°C for 60 min.
2. Denature, reduce and alkylate
Add LYSE reagent, and incubate for 10 mins at 60°C.
Cool, add DIGEST reagent, incubate for 60 mins at 37°C.
Load onto cartridge, WASH and ELUTE. Dry and resuspend in LOAD.
5. MS analysis
Inject and analyze to see improved peptide identities.