cellenONE® X1 NEO

High accuracy single cell isolation and dispensing

cellenONE® is an all-in-one platform combining high-accuracy single cell isolation and high-precision pico to nanoliter reagent dispensing. Using gentle acoustic waves, it offers the most cell-friendly approach, preserving cell viability for cloning and maintaining protein expression for single-cell sequencing applications. With high-resolution optics, cellenONE® can isolate any cell type, from mammalian cells to bacteria, fungi, plants, and more, regardless of sample size.

cellenONE® X1 NEO

cellenONE® X1 NEO

Expanding the horizons of single cell research

Ultra-High Accuracy

Up to 100% single-cell accuracy (no doublet, no debris, no empty well)

Image-Based Technologies

Image-based single cell sorting and isolation

Miniaturization

Miniaturized end-to-end single cell sample preparation workflows

Important Features to Consider

Redefining single-cell proteomics

Radically improved

While cellenONE X1 is now recognized as the gold standard in flexible single cell platforms, with about 200 instruments installed globally, the new version, cellenONE X1 Neo, is even better, offering enhanced features and performance to meet your evolving needs.

  • New heating / cooling system
  • Better temperature control
  • Faster ramping up and down
  • Improved control of the incubation steps
  • New optical system for increased fluorescence sensitivity
  • Improved drop generation system for enhanced droplet stability
  • Faster instrument start-up time
  • New sample holder
  • Allowing sample cooling
  • New labware format (i.e. Thermo Scientific™ Piko PCR Plate 24-well , Piko PCR Plate 96-well, and 8-tube stripes)
  • Application-dedicated software packages (e.g. proteomics, cloning, microbiology etc.)
  • Improved user experience
  • Automation of end-to-end sample preparation workflows.

Benefits

Accuracy

Up to 100% single cell accuracy (no doublet, no debris, no empty well)

Viability

Ultra gentle acoustic-based dispensing technology

On-deck sample temperature control for improved cell viability

Image-Based Technology

Live imaging of cell sample content (e.g. diameter, elongation)

User-defined sorting parameters (morphology and/or fluorescence based)

Image-based QC for every isolated cell

Automation

Improved drop generation system

Automated end-to-end run creation and error handling

Fast temperature ramping up and down (4 to 85°C) for efficient sample incubation

Miniaturization

High-precision pico to nanoliter reagent dispensing

On-deck temperature, humidity and dew-point control

Ideal for Rare Cells

Compatible with sample containing very few cells (e.g. micro-biopsies, CSF fluid)

Compatible with very low sample volume (from ~ 3 μL)

Maximized cell recovery (non-isolated cells are collected for further reprocessing)

Versatility

Any cell type (from 0.5 to ~80 μm)

Any sample size (from ~ 3 μL)

Any standard (i.e. 96, 384, 1536 well plates, glass slides, etc.) or custom labware

Time & Cost Saving

96 cells isolated in less than 3 minutes

Workflow miniaturization, reducing reagent costs

Workflow automation, reducing operator time

Applications

Product Specification

Sorting Technology Brightfield and/or Multi-fluorescence cell images (Blue, Green, Orange and Red) images DAPI (ex.375nm; em. 432nm); FITC (ex.490nm; em.515nm); Cy3 (ex.565nm; em.580nm); Cy5 (ex. 625nm; em.670nm)
Dispensing Technology sciDROP PICO: 250-600 pL/single drop
Target Capacity 2 MTPs or 8 standard glass slides
Axis System X-Y Linear Magnetic Drive, Z Spindle Drive
Axis Precision < 3 µm
Dimensions with Enclosure (L x W x H) 650 x 700 x 1590 mm
Weight  242 kg

System Components

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