neofleX™

Contextualizing molecular expression

Experience the future of mass spectrometry with the neofleX™ – the ultimate MALDI-TOF/TOF system that redefines precision and versatility. Transform your lab’s capabilities with this powerful instrument, designed for high-throughput, spatially resolved biomolecule analysis. Seamlessly navigate through complex samples with advanced imaging, intuitive software, and rapid data acquisition. Whether you’re exploring proteomics, metabolomics, or glycomics, the neofleX™ delivers unmatched performance and accuracy, making groundbreaking discoveries routine.
Elevate your research and make the invisible visible with neofleX™.
neofleX™

Key Features

  • True pixel imaging: Patented smartbeam 3D technology delivers industry-standard 20 μm spatial resolution for detailed analysis.
  • Enhanced imaging detectors: Capture high-quality data for accurate results.
  • Sweeping MS/MS capabilities (LID, CID, ISD): More insight into structural details. Seamless top-down and bottom-up sequencing of proteins
  • Seamless Integration with: the rapifleX, the timsTOF fleX and the scimaX

MALDI TOF/TOF

Inspired innovation for axial TOF mass spectrometry

Easy-to-use

An ecosystem of consumables and software

Illuminate

More insight per pixel for contextualizing tissue biology

Rediscover

Enhanced speed and and performance in flexible MALDI workflows

Transform

Multiomic spatial biology applications from one slide

Important Features to Consider

Record breaking simultaneous imaging of 116 antibody probes

By leveraging MALDI Imaging add depth to the multiomic information from a single tissue section. Push your spatial biology further to understand the complex ecosystem of the tumor microenvironment. Ultimately, transform molecular pathway awareness into disease related insights with MALDI HiPLEX-IHC and other multiomic measurements from one tissue sample combined in a single software solution.

Our spatial biology toolkit empowers you to identify targets using disease specific HiPLEX experiments to assess target engagement effects. A groundbreaking 116-plex MALDI HiPLEX-IHC experiment with 30 µm spatial resolution was collected in 7 hours on squamous cell carcinoma. Comprehensive protein analysis enables differentiation of control tissue from small cell lung cancer types.

More Insight Per Pixel

SCiLS™ Scope makes data transfer easy with automated import and viewing as OME-TIFF 

The neofleX™ Imaging Profiler empowers researchers to dig deeper into the molecular expression profiles of tissue, accelerating translational research.

  • Time to Image – automated setup and image import provides ease of use for curated assays
  • Time to Insight – from simplified viewing of key targets to advanced software for multimodal integration and statistical evaluation, SCiLS™ provides options for every user

Applications

Accelerating biologics development

Instant decision making is of critical importance in biopharma. Fast MALDI analysis speed and short time to result offer unique potential for accelerating the biologics development process at various stages.

MALDI Top-Down Sequencing (MALDI-TDS) provides instant information regarding primary sequence, terminal status and near-terminal modifications of pharmaceutical proteins, and has a long history of success in the characterization of therapeutic antibodies, PEGylated proteins, antigens and other modalities.

MALDI-TDS brings unique advantages to particularly challenging analyses, for example sequencing of hydrophobic small membrane proteins, which are often overlooked in proteomics studies, but, nevertheless, represent unique drug targ

Sequence verification of NIST mAB light chain by MALDI-TDS. Data analysis using the Confirmation workflow featured in Bruker OmniScape™ software requires only few mouseclicks and provides confident sequence confirmation at unparalleled coverage with shortest time to result.
Automated batch QC analysis of synthetic peptides from a 384 microtiter plate using the multi-attribute monitoring (MAM) data analysis workflow in BioPharma Compass® software. 

neofleX™ is the perfect tool to provide instant answers to urgent questions in routine applications requiring higher throughput. When integrated with Bruker’s BioPharma Compass® software solution, it’s never been easier to generate fast and meaningful QC results for a broad spectrum of biomolecules including synthetic peptides, intact or digested proteins, glycans, DNA and RNA oligonucleotides.

neofleX™ MALDI-TOF/TOF redefines the meaning of benchtop MS/MS performance. Its newly developed TOF/TOF technology helps reveal structural details, such as modifications or mutations in proteins, which play a vital role in biological pathways, and are key factors to the quality, safety and efficacy of protein drugs.

MALDI-MS/MS spectra of amyloid beta peptide [1-40] (left) and NIST mAB N-glycopeptide EEQYNSTYR/G1F (right). Data analysis was performed in BioPharma Compass® software. 

Software

A complete MALDI Imaging workflow driven by SCiLS™ autopilot

SCiLS® autopilot provides an automated workflow to facilitate sample tracking and imaging run setup and optimization. No experts needed as instrument parameters are optimized ensuring run-to-run reproducibility and readiness. Datafiles from neofleX™ are portable OME-TIFF format for effortless collaboration.

Software driven solutions for MALDI characterization

BioPharma Compass® to simplify routine workflows through automation to increase productivity.

OmniScape™ provides a comprehensive suite for the analysis of top-down data, including de novo protein ID and validation tools for PTM localization.

Consumables

MALDI Imaging consumables

IntelliSlides®: Maximize insight per pixel with intelligent slide technology

Bruker’s IntelliSlides® are the perfect tool for SpatialOMx®. They simplify your MALDI Imaging workflows by enabling automated setup of each measurement and via barcoding and permanent inscriptions on the conductive slide surface for optimal placement of samples and registration marks (teach marks) for imaging.

fleXmatrix® – The key to success in MALDI MS analyses

fleXmatrix® pre-portioned vials simplify matrix solution preparation for MALDI Imaging, especially for standard spraying or sublimation methods. This ensures consistency and saves time in your workflow.

Trusted MALDI consumables for measurement without compromise

AnchorChip Target Technology – Simplify sample preparation and improve reproducibility

Sample positions on patented Bruker AnchorChip targets contain “anchors” – hydrophilic patches surrounded by a hydrophobic ring. The “anchor” localizes droplets and ensures that every single laser shot fired throughout an automatic run will hit a sample spot.

System Components

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