rapifleX® MALDI PharmaPulse®
High-Throughput Screening | Unique speed and specificity offered by label-free MALDI-TOF mass spectrometry
The all new rapifleX® MALDI Time-of-Flight mass spectrometer is the heart of the MALDI PharmaPulse®, it incorporates Brukers latest 10 kHz scanning laser technology and can read multiple samples per second. All components of the system work in concert for fully automatic HTS operation. The newly designed screening software suite supports HTS workflows and works in combination with a proprietary assay development module which empowers assay developer to accelerate label-free assay development for MALDI mass spectrometry.
MALDI-TOF/TOF
rapifleX® MALDI PharmaPulse®
Speed
Less than a second per sample.
Robustness
Maximum uptime in 24/7 operation and full automation.
Confidence
Label-free mass spectrometry for lowest false discovery rates.
Flexibility
Enhancing performance for a wide range of screening applications.
Important Features to Consider
rapifleX® MALDI PharmaPulse®
The all new rapifleX® MALDI Time-of-Flight mass spectrometer is the heart of the MALDI PharmaPulse®, it incorporates Brukers latest 10 kHz scanning laser technology and can read multiple samples per second. All components of the system work in concert for fully automatic HTS operation. The newly designed screening software suite supports HTS workflows and works in combination with a proprietary assay development module which empowers assay developer to accelerate label-free assay development for MALDI mass spectrometry. The latest ‚Synthesis Screening‘ software module of the rapifleX® MPP opens a new application space for e.g. monitoring of chemical reactions at high pace or analysis of binding assays at incomparable high throughput and specificity.
rapifleX® MALDI PharmaPulse® (MPP) combs through large compound libraries of millions of substances
The use of mass spectrometry allows the measurement of unmodified substrates in primary screens to avoid false positives or false negatives, making confirmation screens redundant. The system is designed for fully automatic handling of 384 and 1536 sample plates to screen more than a million compounds in a week in support of drug discovery.
New standard for ultra-High-Throughput Screening Label-Free ‘Hit’ Identification
By combining the speed of Bruker’s patented smartbeam 3D laser technology with the direct-detection capability of mass spectrometry, the rapifleX® MPP delivers unprecedented screening capability for ultra high-throughput screening of biochemical and cellular assays. It allows you to screen through large and diverse compound libraries at a pace of over a million compounds a week, epitomizing the perfect balance of automation and miniaturization.
Robustness to analyze 2-3 million samples without intervention
- New source and ion optical design
- No source/ion optic cleaning even within primary screens
- Easy and fast cleaning if required
Compatible with CyBio®-Well vario with 384 or 1536 channel simultaneous pipettor for high precision and ultra-fast sample plate preparation
Single digit cost per sample
- No LC solvents, no waste, no labeling
- 0.025 to 1 μL sample volume
- Low cost disposable sample plates
Fully automatic 1536 sample plate change
- New ultra-fast autoloader seamlessly integrable with common robot systems
- Rugged design tailored for uHTS operation
New disposable MALDI HTS sample plates
- Fulfills all requirements for reliable MALDI ionization
- Suitable for 384, 1536, 3072 or 6144 geometries
Benefits
The rapifleX® MPP assists assay development, accelerates HTS and uHTS hit finding, and supports hit confirmation and lead optimization
The MPP system allows for the measurement of unmodified substrates in primary biochemical screens, and offers several advantages:
PharmaPulse Solution
The PharmaPulse Solution is based on Bruker´s advanced MALDI-TOF technology and on a dedicated software package. Ease of use and time to result were key drivers during the development resulting in an intuitive but yet powerful software solution to measure and analyze millions of samples in full automation. Two workflows guide the user from adapting an assay to MALDI mass spectrometry to readily exported results for further analysis in LIMS systems.
New software module supports synthesis screening and binding assays
In biochemical screening experiments, the rapifleX® MPP determines the rate of conversion in a biochemical assay when performed in the presence of potential inhibitors. In such a screen, a distinct MS peak ratio comparing product and substrate intensities is monitored throughout large numbers of sample spots, where each spot represents the same biochemical conversion reaction, but measures the effectiveness of a different inhibitor molecule.
In addition to this screening functionality, the new MPP software module supports “Synthesis Screening”, a methodology that requires more flexibility with regard to m/z values to be targeted by MALDI-TOF MS. Here, m/z values of interest may vary from spot to spot as each sample may represent a different chemical reaction. Accordingly, the new MPP “Synthesis Screening” software module allows scientists to screen for a large number of compounds per individual sample spot. This new MALDI-TOF based high-throughput analysis workflow could, therefore, enable highly time-efficient surveying of chemical reactivity landscapes at previously unseen mapping depth.
Applications
rapifleX® MPP – the universal tool for drug discovery
Full library screens
The rapifleX® MPP is the only mass spectrometer for large primary screens of millions of compounds with high diversity. Full automation supports screening of more than one million compounds per week, avoiding time-consuming confirmatory screens. [1]
Focused library screens
The quality of data obtained with rapifleX® MPP meets the requirements of secondary and confirmatory screens and makes it the ideal detector for focused library screens, such as the identification of kinase inhibitors of a target protein from a smaller subset of the library. [2]
Fragment screening
The mass range and resolution accessible by MALDI-TOF mass spectrometry enables screening of fragments and other low molecular weight compounds. [3]
[1] Winter, M.; Ries, R.; Kleiner, C.; Bischoff, D.; Luippold, A. H.; Bretschneider, T.; Buettner, F. H.; Automated MALDI Target Preparation Concept: Providing Ultra-High-Throughput Mass Spectrometry–Based Screening for Drug Discovery. SLAS Technology 2018, https://doi.org/10.1177/2472630318791981
[2] Beeman, K.; Baumgartner, J.; Laubenheimer, M.; Hergesell, K.; Hoffmann, M.; Pehl, U.; Fischer, F.; Pieck, J.C.; Integration of an In Situ MALDI-Based High-Throughput Screening Process: A Case Study with Receptor Tyrosine Kinase c-MET. SLAS Disc. 2017, 22, 1203–1210.
[3] VanderPorten, E.; Scholle, M.; Sherrill, J.; et al. Identification of Small-Molecule Noncovalent Binders Utilizing SAMDI Technology. SLAS Discov. 2017, 22(10):1211-1217.
Target evaluation and hit confirmation
Thorough understanding of enzyme biochemistry and kinetics is essential to develop and validate robust HTS assays. rapiflex® MPP assists assay development and hit confirmation with linearity tests, substrate selection, Ki measurements and IC50 determination.
MPP Software: Settings Perspective
MPP Software: Visualize Perspective
IC50 determination of inhibitors
Half-maximal inhibitor concentration (IC50) is a key parameter in the drug discovery process. MALDI PharmaPulse® software supports assay development by featuring a dedicated method development module. Optimal assay conditions obtained using the method development module are applied during MALDI-TOF screening, for example aiming for IC50 determination, which is set up and executed in the software´s dedicated screening module.
rapifleX® MPP – for primary screening
Drug Discovery workflow with rapiflex MALDI PharmaPulse
The rapifleX MALDI PharmaPulse offers the use of Mass Spectrometry for large screening campaigns
A million samples per week with rapifleX® MALDI PharmaPulse®
The new rapifleX® MPP is the result of Bruker’s innovative development of MALDI mass spectrometers. It reflects our passion for and knowledge about this technology and its unique applications. The time-of-flight mass spectrometer (TOF MS) of the rapifleX® MALDI PharmaPulse® monitors up to 30 masses in HTS mode: from small molecules to large proteins. The improved resolution and sensitivity of the rapifleX® TOF MS allows e.g. the monitoring of acetylcholine (ACh) and choline to identify acetylcholinesterase (AChE) inhibitors out of millions of substances. An ingenious detector design delivers significantly improved signal to noise ratios for enhanced sensitivity across the mass range. A completely new HTS software suite supports easy set-up of HTS screens, and the proprietary assay development module ensures fast adaption of label-free assays for MALDI mass spectrometry.
Automation of rapifleX® MALDI PharmaPulse®
The rapifleX® MALDI PharmaPulse® is designed to seamlessly integrate with laboratory automation systems to perform fully automatic screening campaigns. The MALDI PharmaPulse® software solution offers an open interface to control parts of its operation from external scheduling software (e.g. Analytik Jena lab automation, HighRes Biosolutions, Beckman Coulter, Thermo Scientific automation). The new high-speed autoloader of the rapifleX® MPP works hand in hand with robot systems for quick loading and unloading of sample plates.
Acetylcholinesterase
