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spheroONE – Automated sorting and isolation of single spheroids and organoids

spheroONE is a cutting-edge platform for sorting and dispensing individual large particles, transforming the handling of 3D cellular models. Combining precise dispensing technology with advanced image-based selection, it enables the efficient isolation of single spheroids, organoids, and tumoroids. spheroONE is an essential tool for drug screening and other applications where 3D cellular models are increasingly replacing traditional animal testing.

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spheroONE

Key Features

Dispense Volume 30 – 800 pL/drop
No. of Dispense Capillaries From 1 to 8 PDC channels
Distance Dispense Capillaries 4.5 or 9 mm increments
Target Holder 380 x 380 mm
Capacity Up to 12 MTPs or 70 standard glass slides
Axis system X-Y linear magnetic & Z spindle drives
Resolution (step size) 1 µm
Precision < 3 µm
Accuracy < 5 µm
Options Spot-on-the-fly, 3D drop camera, vacuum target holder, online target alignment, online array QC software
Dimensions (L,W,H) with enclosure 1750 x 770 x 1200 mm
Weight 420 kg

spheroONE Benefits

  • Enables fully automated isolation of single cellular aggregates with up to 100% accuracy, using size and shape-based morphological sorting
  • Handles particles ranging from 80 to 600 µm in diameter
  • Gentle dispensing preserves the integrity and viability of delicate cellular aggregates, such as organoids
  • Allows direct dispensing and positioning of aggregates into Matrigel® or alternative ECMs (e.g., HyStem)
  • Provides precise control over biomass per well, supporting user-defined numbers of spheroids and producing highly uniform populations
  • Efficiently removes cellular debris to improve imaging quality and enhance drug screening outcomes
  • Compatible with standard multiwell plates (96- or 384-well) as well as custom labware
  • Offers direct visual inspection of samples along with a complete image record of each isolated aggregate

spheroONE Applications

Complex In – Vitro 3D Models
Stem Cells
Drug Discovery

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