timsMetabo™

Discover MoRE with Mobility Range Enhanced 4D-Metabolomics™

The timsMetabo™ by Bruker is a powerhouse 4D‑Metabolomics™ and 4D‑Lipidomics™ platform, built on the award‑winning timsTOF architecture. It combines Trapped Ion Mobility Spectrometry (TIMS) with MoRE scan‑mode and Athena Ion Processor, achieving game‑changing sensitivity, selectivity, and broad m/z coverage for small molecules.

Its real‑time CCS measurements and LC‑TIMS‑MS/MS workflow resolve isomers/isobars at speed and scale, building a “digital metabolome archive” ideal for AI‑driven annotation. With integrated QSee/TwinScape QC and support for HESI/CSI sources, it offers laboratory confidence and versatility—perfect for drug discovery, biomarker profiling, environmental, and lipid research.

timsMetabo™

Key Features

  • Game-changing sensitivity for TIMS-enabled small molecule analysis
  • 4D LC-TIMS-MS/MS separations and CCS measurements at scale for unprecedented specificity and annotation confidence
  • TIMS-enabled confidence in automated analyte annotation
  • A digital metabolome archive of every sample analyzed
  • Powerful 4D-Lipidomics and 4D-Proteomics capabilities
  • Fully featured 4D-enabled quality control and established software ecosystem

4D-Metabolomics™ and 4D-Lipidomics™

For unprecedented sensitivity, separation power and annotation confidence for small molecules.

A digital metabolome archive of every sample analyzed

Simultaneous quantitation and characterization of analytes within each sample virtually eliminates the need for re-runs.

Routine 4D separations and CCS measurements enable confident annotation

See MoRE metabolism thanks to the novel TIMS Mobility Range Enhancement (MoRE) technology and established MetaboScape® software.

Fully featured 4D-enabled quality control and established software ecosystem

Longitudinal monitoring of key performance attributes across platforms with TASQ® RealTimeQC, QSee™ performance testing, and TwinScape™.

4D-Metabolomics™ and 4D-Lipidomics™ at speed, at depth, and at scale

This benchtop platform brings together technological innovations for uncompromised small-molecule performance.

Discover the advantages:

TIMS-MX and Mobility Range Enhancement (MoRE) leverage mobility-based ion sorting to increase the separation capacity and enable confident metabolite annotation.
The Athena Ion Processor (AIP) works synergistically with TIMS-MX to boost MS and MS/MS sensitivity, helping to detect, measure, and annotate low-abundance compounds in complex samples.
The VIP-HESI ion source provides maximum signal intensity and sensitivity.
The precision detection system supports reliable profiling and quantitation of metabolites and lipids.

The power to see MoRE metabolism

The timsMetabo™’s enhanced sensitivity enables deeper and more accurate profiling with less sample. A refined ion path geometry builds on successive generations of timsTOF design, enabling efficient ion sampling for incredible sensitivity with analytical scale LC compatibility.

The timsUltra AIP: Unmatched performance for deep proteome analysis

TIMS-enhancement enables resolution of isomers, isobars and interferences, reducing the incidence of chimeric MS/MS spectra for high-confidence automated annotation. Lipid and metabolite annotation benefits from clean MS/MS spectra, especially in automated downstream workflows utilising spectral libraries and in-silico fragmentation (4D-Metabolomics™) and rule-based annotation (4D-Lipidomics™). Here, co-eluting and co-isolated lipid species, measured using dia-PASEF® 4D-Lipidomics™, are mobility separated, producing distinctive and characteristic MS/MS fragmentation spectra for each. These cleaned spectra form the cornerstone of accurate annotation, interpretation and reporting.

Extended mobility capability for 4D-Metabolomics™

The TIMS-MX analyzer and MoRE enhance small molecule transmission for broad metabolome coverage in 4D-Metabolomics™ workflows.

Achieve accurate quantitation with low limits of detection for small molecules while using 4D-Metabolomics™ workflows that simultaneously and routinely deliver accurate m/z and CCS measurements for high confidence metabolite annotation.

A Digital Metabolome Archive from each sample analysis

The timsMetabo™ enables simultaneous quantitation and characterisation of the analytes within each sample analysis, virtually eliminating the need for re-acquisition. timsMetabo™ data enable confident analysis and annotation, and are well suited for AI in metabolomics with machine learning from large-scale, high-quality data sets.

QSee™ for System Performance Testing

Introducing the QSee™ solution for System Performance Testing.

Ensuring data reproducibility is crucial in scientific research, particularly in untargeted “omics” mass spectrometry. The QSee™ Performance Test Software and the QSee™ 8-Mix reference mix provide a comprehensive solution for automated system suitability testing in LC-TIMS-MS, promoting consistency and accuracy in large cohort studies.

For System Performance Testing: Each QSee™ Test returns a report with a pass or fail feedback
For Long-term Surveillance: Longitudinal data can be used for trend analysis

Data quality monitoring for precision results

When pushing the boundaries of metabolomics and lipidomics-based discovery research, the importance of data quality is paramount. The QSee™ Performance Test Solution offers an easy-to-use Quality Control workflow. Dedicated columns for metabolomics and lipidomics applications, and compound mixtures, together with Standard Operating Procedures simplify the evaluation of LC-TIMS-MS data quality. Automatic alignment with TwinScape™ uncovers trends in system performance over time. Stay on top of your performance!

Advanced profiling and quantification of novel bile acids

Newly discovered, amino acid conjugated bile acids are involved in gut microbe-host interactions. Characterizing these novel and structurally similar compounds is imperative for deciphering their biological function.

Identifying biomarkers of tobacco smoking exposure in human urine

Unknown metabolic features can be annotated using tools for matching experimental and predicted CCS values and MS/MS spectra.

Unlock comprehensive proteomics insights with timsMetabo

timsMetabo™ is designed to excel in lipidomics and metabolomics while preserving the unique proteomics research capabilities of the timsTOF family. By integrating multiomics capabilities in a single platform, this system offers uniquely comprehensive insights into biological processes, enabling researchers to uncover complex molecular interactions and pathways.

System Components

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