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Preparative system for cleanup tasks relying on gel permeation GPC

Automatic cleanup of up to 15 samples

Do not waste time and labor with manual sample preparation. The AZURA® GPC Cleanup system automates work-intensive and time-consuming cleanup tasks based on gel permeation chromatography (GPC). Further, reproducibility and quality of the cleanup are improved. After sample loading, the dedicated system fully automatically processes up to 15 samples while requiring minimal bench space. Based on the versatile AZURA® device platform, the system can be customized to fulfill individual requirements. The cost-effective software Mobile Control provides a touch-optimized user interface for intuitive device control directly at the system.

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Preparative system for cleanup tasks relying on gel permeation GPC

Key Features

System layout

  • GPC Cleanup System can be easily adapted to pre-established laboratory procedures to perform a wide range of sample preparation tasks.
  • The required bench space is kept at a minimum.
  • The system features 15 sample loops controlled by two 16 port multiposition valves.
  • The GPC tubing guide sorts outlet tubings coming from the fraction collection valve.
  • The wound-up sample loops are stored in a tray with drainage system and are easily accessible for inspection and replacement. With an additional multiposition valve 15 fractions and waste can be collected.
  • All multiposition valves and the manual injection valve are integrated into a compact AZURA® Assistant ASM 2.2L.
  • Elution of separated substances/standard components is monitored by a variable single wavelength UV detector.
  • A second assistant harbors the small detector, the system pump with a pressure sensor and a valve to bypass the GPC column or select between two columns.

System control

  • The GPC Cleanup System is operated with the cost-effective software Mobile Control. This clearly arranged and touch-optimized user interface is run on a tablet directly mounted at the system. It automatically recognizes devices and the system is configured within a few finger swipes.
  • Due to the arrangement in functional blocks like eluent delivery, sample injection, detection, fraction collection, programs are created fast with a minimal number of clicks.
  • Mobile Control makes sample loop loading easily manageable by synchronously switching both valves at the push of a button.

Specifications

System type Compact
Gradient Isocratic
Max. flow rate 10 ml/min
Detector Variable single wavelength detection UV/VIS
Injection Manual injection
Fractionation multi-position valve
Software Mobile Control

 

What is GPC Cleanup?

GPC Cleanup (Gel Permeation Chromatography) is primarily employed for performing general cleanup tasks on a wide range of sample matrices such as foodstuffs, tissues, plants and environmental samples. The separation of components takes place according to their molecular weight due to the heterogeneous pore size of the stationary phase which acts as a molecular sieve. High-molecular substances such as lipids, proteins, natural resins, cellular components and steroids interfering with subsequent analysis e.g. of pesticides are efficiently removed.

Separation principle

 

In accordance with established methods to determine pesticide residues

  • Method 984.21 – AOAC international
  • EPA SW-846 Method 3640A – US Environmental Protection Agency
  • AEN 12393 and EN 1528 – European Standard
  • L 00.00-34 Method in accordance with §64 LFGB (formerly § 35 LMBG)

The AZURA® GPC Cleanup system was selected by the Analytical Scientist for “The Innovators 2017” product. Click here to read the full article.

The GPC Cleanup System is also covered in the poster “Automated sample preparation with touch-optimized control software.” Check the downloads below.

System Components

Glass column holder

Glass column holder

Glass column holder, Stand, plate and 2 clamps, can hold one glass column in the dimensions of 10-40 mm ID
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Installation Box Kit

Installation Box Kit

Installation Box Kit, Box for small parts, KNAUER file folder and support sticker
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AZURA Organizer

AZURA Organizer

for attachment of columns and FPLC accessoires to an AZURA device or system
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AZURA Click mounting rail

AZURA Click mounting rail

to attach IFU 2.1 LAN, air sensors, pressure sensors, pH flow cells, or the AZURA® Organizer to AZURA® L devices
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Start-Up Kit for the GPC Cleanup system

Start-Up Kit for the GPC Cleanup system

AZURA GPC Cleanup Start up kit, Tefzel-(ETFE) tubing, OD 1/16", ID 0.7 mm
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AZURA GPC tubing guide with 16 separate sample loops (1 ml)

AZURA GPC tubing guide with 16 separate sample loops (1 ml)

GPC tubing guide for sorting tubings left-sided of AZURA L device for eluent tray AZC00 — 2 rows of each 16 holes with numbered position — with 16 separate sample loops, each 1 ml (Tefzel, OD 1/16“, ID 1 mm)
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Semi-preparative 3 mm UV Flow Cell

Semi-preparative 3 mm UV Flow Cell

3 mm path length, 2 µl, 1/16", stainless steel, classical KNAUER flow cell
Flow cell type: Semi-preparative UV flow cell
Path length: 3 mm
Capillary connection: 1/16''
Max. flow rate: 50 ml/min
Flow cell volume: 2 µl
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Multiposition valve, 16 Port

Multiposition valve, 16 Port

Multiposition valve, 16 Port, DLC stainless steel, POM, 1/16", 100 bar, 0.6 mm bore size
Operated: electrical by VU 4.1
Port number: 16
Positions: Multiposition
Material: Stainless Steel
Capillary connection: 1/16"
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2-position valve, 6 Port

2-position valve, 6 Port

2-position valve, 6 Port, DLC stainless steel, POM, 1/16", 100 bar, 0.75 mm bore size
Operated: electrical by VU 4.1
Port number: 6
Positions: 2
Material: Stainless Steel
Capillary connection: 1/16"
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Manual injection valve, 6 Port

Manual injection valve, 6 Port

Manual injection valve, 6 Port, DLC stainless steel, POM, 1/16", 100 bar, 0.75 mm bore size
Operated: manual
Port number: 6
Positions: 2
Material: Stainless Steel
Capillary connection: 1/16"
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Customizable docking station for pumps, valves and detectors – ASM 2.2L

Customizable docking station for pumps, valves and detectors – ASM 2.2L

Left module: UV detector UVD 2.1S - EDA03XA Middle module: Valve drive VU 4.1 - EWA04 Right module: Pump P 4.1S, 10 ml, stainless steel - DPG22EA
Left module: UV detector UVD 2.1S - EDA03XA
Middle module: Valve drive VU 4.1 - EWA04
Right module: Pump P 4.1S, 10 ml, stainless steel - DPG22EA
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Display software for AZURA devices with data acquisition incl. tablet

Display software for AZURA devices with data acquisition incl. tablet

Mobile Control Chrom with data acquisition, including tablet and mount
Software name: Mobile Control Chrom - display software for AZURA devices with data acquisition
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Mounting bracket AZURA L

Mounting bracket AZURA L

Mounting bracket AZURA® L for KNAUER manual injection valves
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Customizable docking station for pumps, valves and detectors – ASM 2.2L

Customizable docking station for pumps, valves and detectors – ASM 2.2L

Left module: UV detector UVD 2.1S - EDA03XA
Middle module: Empty module
Right module: Pump P 4.1S, 10 ml, stainless steel - DPG22EA
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WiFi router, 8x GBit RJ-45 ports, for connection of AZURA devices with a PC

WiFi router, 8x GBit RJ-45 ports, for connection of AZURA devices with a PC

WiFi router, 8x LAN GBit RJ-45 ports, 1x WAN GBit RJ-45 port
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AZURA Eluent tray E 2.1L

AZURA Eluent tray E 2.1L

AZURA® Eluent tray E 2.1L for AZURA® devices with a capacity of 6 x 1 l bottles or 4 x 2.5 l bottles or 2 x 5 l bottles, (delivery without bottles)
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Downloads

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