Home > Products > Scientific Instruments > Liquid Chromatography Systems > Fast Protein Liquid Chromatography (FPLC) Systems > Gradient capable FPLC system for complex applications

Gradient capable FPLC system

for complex applications including automated two step applications

for up to 50 mL/min

The AZURA Bio Lab System Advanced is a gradient system for complex purifications of bio molecules in a laboratory scale. It is designed and optimized for all FPLC applications like affinity, ion exchange, hydrophobic interaction and size exclusion chromatography. All system modules can be freely exchanged and further modules can be added optionally, to give you the best configuration for your purification task.

In TwoStep mode, a column selection valve will allow you to switch between a multitude of different columns, which in combination with an optional outlet valve allows even for automated two step purifications.

Due to its modular design, the system can be easily up scaled to higher flow rates by simply replacing the pump head, valves, flow cell and capillaries. The basic devices do not need to be replaced. The next scale-up level allows flow rates up to 200 ml/min at 200 bar. The 1/16″ capillaries are exchanged for 1/8″ capillaries. Higher sample volumes are injected via a channel of the gradient pump. The modules of the HPLC docking station and the valves are exchanged within minutes and allow to flexibly adapt the system to changing purification tasks.

Request a Quote
Contact Support
Gradient capable FPLC system for complex applications

Key Features

  • Flow rate: 0.001–50 ml/min; 0.1 – 40 ml/min (recommended)
  • Maximum system pressure: 240 bar (24.0 MPa)
  • Injection valve for sample injection via sample loop or sample pump
  • Sample pump, 0.1 – 50 ml/min
  • Variable multi-wavelength UV detector (190–700 nm)
  • Conductivity monitor with pH option
  • Fraction collector for fractionation
  • Compatible with columns from all vendors
  • Compatible with SEC, AC, IEX and HIC methods
  • Intuitive PurityChrom® software
  • System suitable for cold room usage

Specifications

Purification complex, challenging
Application suitable for all
Gradient Yes
Flow rate 50 ml/min

System Components

Conductivity monitor with flow cell for up to 100 ml/min and optional pH measurement

Conductivity monitor with flow cell for up to 100 ml/min and optional pH measurement

AZURA CM 2.1S with flow cell - up to 100 ml/min - conductivity monitor with optional pH measurement
Detector type: Conductivity monitor
Measurement range, Conductivity: 0.1 - 999 mS/cm
pH: 0 - 14
Linearity, Conductivity: ± 1 % full scale value (0.1 - 300 mS/cm), ± 1 % full scale value (300 - 999 mS/cm)
View Component
AZURA pH sensor, 100 ml/min

AZURA pH sensor, 100 ml/min

for conductivity, monitor CM 2.1S
Capillary connection : 1/16", 1/8"
Max. flow rate: 100 ml/min
Flow cell volume: ~ 80 µl (with inserted eclectrode or dummy electrode)
Maximum pressure: 5 bar
Biocompatible: Yes
View Component
Pulse Damper, High Volume, PEEK

Pulse Damper, High Volume, PEEK

KNAUER Pulse Damper, 290 µl, PEEK, 1/16'', 225 bar
View Component
Air Sensor

Air Sensor

Air sensor (1/16") for AZURA® Bio LC with one air sensor and wiring for up to 4 air sensors
View Component
Smart valve drive with RFID-technology

Smart valve drive with RFID-technology

VU 4.1 valve drive for V 4.1 valves
View Component
Biocompatible column selction valve

Biocompatible column selction valve

Column Selection Valve VICI for 5 columns, 1 bypass, reverse flow, 12 ports, 1/16“, 50 bar
Operated: electrical by VU 4.1
Port number: 12
Positions: Multiposition
Material: Biocompatible
Capillary connection: 1/16"
View Component
Pressure Control Set

Pressure Control Set

Pressure control for delta pressure measurement up to 250 ml/min for 1/16'' and 1/8''. Includes interface box.
View Component
Mounting bracket

Mounting bracket

Mounting bracket AZURA® L for AZURA® Valve Unifier VU 4.1, AZURA® Conductivity monitor CM 2.1S, AZURA® Degasser DG 2.1S or AZURA® UV detector UVD 2.1S on AZURA® L devices
View Component
Multi-injection valve, biocompatible

Multi-injection valve, biocompatible

Especially for FPLC customers: New biocompatible multi-injection valve for more sophisticated separation tasks. (PEEK, 1/16", 240 bar, 0.75 mm bore size)
Operated: electrical by VU 4.1
Port number: 8
Positions: Multiposition
Material: Biocompatible
Capillary connection: 1/16"
View Component
Biocompatible multiposition valve, 8 Port, break-free rotor seal

Biocompatible multiposition valve, 8 Port, break-free rotor seal

Multiposition valve, 8 Port, PEEK, PEEK, 1/16'', 240 bar, 0.75 mm bore size, with break-free valve design
Operated: electrical by VU 4.1
Port number: 8
Positions : Multiposition
Material: Biocompatible
Capillary connection: 1/16"
View Component
Customizable docking station for pumps, valves and detectors – ASM 2.2L

Customizable docking station for pumps, valves and detectors – ASM 2.2L

Left module: Valve drive VU 4.1 - EWA04 Middle module: Pump P 4.1S, 50 bar, 50 ml, ceramic - DPG12FB Right module: Valve drive VU 4.1 - EWA04
Left module: Valve drive VU 4.1 - EWA04
Middle module: Pump P 4.1S, 50 bar, 50 ml, ceramic - DPG12FB
Right module: Valve drive VU 4.1 - EWA04
View Component
High-pressure gradient FPLC pump for semi-preparative tasks

High-pressure gradient FPLC pump for semi-preparative tasks

AZURA® Pump P 6.1L (HPG), without degasser, with 50 ml pump head (ceramic) and mixer (250 µl)
Max. flow rate: 50 ml/min
Pump head materials: Ceramic
Gradient: High-pressure gradient (binary)
Pump features: Active pulsation compensation, RFID for automatic pump head detection, Integrated pressure sensor, Purge valve (manual), Leak management, Piston seal wash
Maximum delivery pressure [MPa]: 20 MPa
View Component
Semi-preparative KNAUER PressureProof UV Flow Cell Cartridge

Semi-preparative KNAUER PressureProof UV Flow Cell Cartridge

3 mm path length, 2µl, 1/16", 300 bar, PressureProof Flow cell cartridge for AZURA® Detector DAD/MWD
Flow cell type: Semi-preparative UV flow cell
Path length: 3 mm
Capillary connection : 1/16''
Max. flow rate: 100 ml/min
Flow cell volume: 2 µl
View Component
Fraction Collector Foxy R1

Fraction Collector Foxy R1

Fraction collector Foxy® R1 for 1/16“ or 1/8“ tubing
Max. flow rate: 25 ml/min or 125 ml/min
Capillary connection: 1/16'' or 1/8''
Brand: Foxy R1
View Component
Rack for Foxy R1/R2

Rack for Foxy R1/R2

Rack for 72 Falcons 15 ml for Foxy® R1/R2
View Component
PurityChrom® Basic

PurityChrom® Basic

Basic License for one system
Software name: PurityChrom® 5
View Component
PurityChrom® 5 Upgrade

PurityChrom® 5 Upgrade

Extends the Basic License to an unlimited number of controllable devices and 8 data channels, adds autosampler and stacked injections support
Software name: PurityChrom® 5
View Component
8-port GBit switch for connection of AZURA devices with a PC

8-port GBit switch for connection of AZURA devices with a PC

8-port LAN GBit switch NetGear GS108GE, 8x RJ-45, GBit, Auto MDI-X, power plug UK, US or AUS
View Component
Backpressure Regulator

Backpressure Regulator

Backpressure Regulator for 1/16“ OD tubing, 10-32 threads, PEEK, Range 1-20 bar (15-300 psi)
View Component
AZURA Organizer

AZURA Organizer

for attachment of columns and FPLC accessoires to an AZURA device or system
View Component
Versatile Multi Wavelength UV-Detector for Liquid Chromatography and Process Technology

Versatile Multi Wavelength UV-Detector for Liquid Chromatography and Process Technology

AZURA® Detector MWD 2.1L Multiwavelength detector MWD 2.1L, without flow cell 190 - 700 nm, incl. test cell
Detector type: Variable multiwavelength detector
Detection channels: 8 (Digital)/4 (Analog)
Wavelength range: 190 - 700 nm
Linearity : > 2.0 AU at 274 nm (ASTM E1657-98)
View Component
AZURA Eluent tray E 2.1L

AZURA Eluent tray E 2.1L

AZURA® Eluent tray E 2.1L for AZURA® devices with a capacity of 6 x 1 l bottles or 4 x 2.5 l bottles or 2 x 5 l bottles, (delivery without bottles)
View Component

Downloads

Related Products

No items found

#wecanhelp_

"A great product can last forever"

Scroll to Top